Lisa Strasser

Department Molecular Biology 
PhD Supervisor Univ. Prof. Mag. Dr. Christian Huber
PhD Co-Supervisor Dr. Roland Reischl
Start WS 2014/15
Contact Lisa.Strasser(at)sbg.ac.at
Doctoral Thesis Topic Proteomic profiling of stimulated monocyte derived dendritic cells

Abstract

As antigen presenting cells, especially dendritic cells (DCs), are crucial players in the initiation phase of an allergic immune response. Therefore, we analyze the proteome of monocyte derived dendritic cells (moDCs) after stimulation with birch pollen extract (betex) and a recombinant variant of the major birch pollen allergen Bet v 1 (rBet). Thus, we try to elucidate, whether an allergic immune response is triggered by the allergen itself or if the combination of stimulants contained in the pollen (e.g. endotoxins) is required.

To address this question we perform quantitative proteome analysis using iTRAQ®-labeling for relative quantification. Hence, we generate moDCs and stimulate them with betex [10.0 µg/ml], rBet [10 µg/ml] and lipopolysaccharide [1.0 ng/ml]. Subsequently, proteins are extracted and tryptically digested. To reduce donor variability, peptides of several donors are merged prior to the labeling. Samples are then analyzed using reversed-phase (RP) high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS).

  • News
    Sharing in favor of ArMut - Helping single parents in acute financial emergencies.
    On Wednesday, the 27th of November, 2019, the University of Salzburg warmly congratulated 56 graduates who celebrated their graduations and/or completions of doctoral theses.
    Once again, the Universitätsbibliothek Salzburg will be opening the Advent 24 Windows in the Hofstallgasse and in the online Advent calendar. Texts and images may "brighten your inspiration" in time for Christmas.
    The physician and psychologist Hendrik Lehnert was solemnly introduced to his position as Rector of the Paris Lodron University Salzburg on FR 22.11.19. The new rector will be assisted by materials chemist, Nicola Hüsing; innovation expert, Katja Hutter; financial expert, Barbara Romauer; and sociologist, Martin Weichbold. Click here for Photo Gallery
  • Veranstaltungen
  • 16.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
    16.12.19 Ukraine – Moskau – Konstantinopel
    17.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
    18.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
    19.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
    19.12.19 Salzburger Museen und Sammlungen Geschichte vor Ort. Sozialanthropologische Lehrforschung als partizipatives Projekt mit Museum und Gemeinde
    20.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
    21.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
    22.12.19 Lichtvolle Impulse in der vorweihnachtlichen Zeit
  • Alumni Club
  • PRESS
  • Uni-Shop
  • University of Salzburg's facebook site University of Salzburg's twitter site University of Salzburg's instagram site University of Salzburg's flickr site University of Salzburg's vimeo site