Sabrina Wildner

Department Molecular Biology 
PhD Supervisor Univ. Prof. Dr. Fatima Ferreira
PhD Co-Supervisor Dr. Gabriele Gadermaier
Start SS 14
Contact sabrina.wildner(at)sbg.ac.at
Doctoral Thesis Topic Structural and immunological characterization of biosimilars and allergens

Abstract

Biopharmaceuticals (biologics) are protein molecules produced in living cells and used in therapy for a wide range of disorders like cancer, autoimmune or autoinflammatory diseases. In contrast to small molecule drugs, biologics are highly sensitive to their manufacturing and handling conditions. The production of biopharmaceutical drugs in living cells can thus result in minor conformational differences due to posttranslational modifications, different codon usage, etc. These subtle conformational changes can however impact the safety, efficacy and stability of the product and can remain undetected by analytical methods. A new tool termed aptamers can help to detect changes in three-dimensional structures of biopharmaceutical products. Aptamers are short, single-stranded oligonucleotides which can adopt specific three dimensional shapes. SELEX (systematic evolution of ligands by exponential enrichment) is a technique for screening large oligonucleotide libraries and is used for in vitro selection and amplification. My aim is to select a panel of aptamers reactive against an antibody-based biopharmaceutical. Aptamers can be used as surrogate antibodies in ELISA assays to characterize potential conformational differences between originator and biosimilar, monitor a production process or determine structural changes during shelf life of a product.

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    The Botanical Garden invites you to a free guided tour! Meeting point: entrance to the Botanical Garden. Duration: about two hours. The tours takes place in all weather conditions. Botanical Garden, Hellbrunnerstrasse 34.
    The 7th Salzburg Summer School will take place the 2nd and 3rd of September, 2019. A comprehensive pedagogical summer education program is offered for teachers and student teachers of all types of schools and subjects. Registrations are possible.
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